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KMID : 0378019750180091149
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New Medical Journal
1975 Volume.18 No. 9 p.1149 ~ p.1153
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Absorption of Ferritin into the Ectodermal Cells in Early Chick Embryos
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Abstract
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In early chick embryos, which were developed to the stage 4, definitive streak stage by Hamburger and Hamilton (1951), a little amount of the ferritin solution (Calchem. San Diego, Calif., 100:ng. / ml.) was injected into the subgerminal cavity by means of micropipette puncturing through the area opaca and then the eggs were reincubated in 37 degrees C. for 2 hours.
The pieces of the blastodisc were carefully removed and fixed in the cold 1% osmic acid in phosphate buffer (pH 7. 2). After the dehydration the pieces were embedded in epon 812 by the method of Luft (1961). The thin sections, stained by the method of Watson (1953), ware examin e by electron microscopy.
Several ferritin particles were found in the vesicles of the cytoplasm of the ectodermal cell which were scattered in the vicinity of the plasma membrane or mitochondria.
Consequently it was speculated that the absorption of the vitellus into the ectodermal cells might be processed as well as the absorption of ferritin into those cells took place in the early chick embryo.
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